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DIETARY N g ‐NITR0‐ l ‐ARGININE INDUCES SUSTAINED HYPERTENSION IN NORMOTENSIVE WISTAR‐KYOTO RATS
Author(s) -
Ikeda K.,
Jr O. G. Gutierrez,
Yamori Y.
Publication year - 1992
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1992.tb00508.x
Subject(s) - nitric oxide , blood pressure , arginine , medicine , endocrinology , nitroarginine , endothelium , hemodynamics , nitric oxide synthase , chemistry , amino acid , biochemistry
SUMMARY 1. Prolonged oral administration of N G ‐nitro‐ l ‐arginine ( l ‐NNA) for a period of 5 weeks in 8 week old male normotensive Wistar‐Kyoto (WKY) rats n = 10), induced hypertension in all animals. Hypertension was characterized by a sharp initial increase in both systolic blood pressure (SBP) and mean blood pressure (MBP) until the third day (from 126 ± 3 mmHg to 160 ± 6 mmHg and from 95 ± 3 mmHg to 133 ± 6 mmHg, respectively). This was followed by a gradual and steady increase until the fourth week (163 ± 4, 171 ± 3 and 189 ± 8 mmHg for SBP in weeks 1, 2 and 4, respectively; and 135 ± 4, 143 ± 3and 157 ±5 mmHg for MBP in weeks 1, 2 and 4, respectively). 2. Intravenously L‐arginine‐HCl (500 mg/kg) administered on the last day of the 5th week abolished the effect of dietary l ‐NNA on the arterial blood pressure. 3. Dietary l ‐NNA‐induced hypertension in WKY rats is easily obtainable and free of any surgical operation, and can be utilized as a new experimental model to further understand the importance of endothelium‐dependent relaxing factor/nitric oxide in blood pressure regulation and to clarify the pathological significance in intact animals where endothelium‐dependent relaxing factor/nitric oxide is functionally involved.