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THE HETEROGENEITY OF 11‐β‐HYDROXYSTEROID DEHYDROGENASE ACTIVITIES IN THE RAT HIPPOCAMPUS IMPLIES A COMPLEX REGULATION OF STEROID HORMONE ACTION
Author(s) -
Smith R. E.,
Mercer W. R.,
Provencher P. H.,
Obeyesekere V.,
Krozowski Z. S.
Publication year - 1992
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1992.tb00473.x
Subject(s) - hippocampal formation , hippocampus , nuclease , complementary dna , gene isoform , messenger rna , biology , medicine , endocrinology , steroid hormone , microbiology and biotechnology , biochemistry , hormone , enzyme , gene
SUMMARY 1. The rat hippocampus 1l‐β‐hydroxysteroid dehydrogenase (11‐HSD) displays a different substrate specificity to that of other tissues. S1 nuclease analysis was used to determine whether the hippocampal messenger RNA is different from that found in other tissues. 2. SI nuclease analysis using probes spanning the full length cDNA demonstrated that there were no differences in sequence between the hippocampal 11‐HSD and the enzyme originally cloned from the liver. 3. These results suggest that there may be multiple 11‐HSD isoforms in the hippocampus with different substrate specificities.