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METABOLIC BASIS OF CATECHOLAMINE‐INDUCED WATER TRANSPORT IN EVERTED GUT SACS OF MOUSE
Author(s) -
Mary P. L.,
Rao J. Prakasa
Publication year - 1991
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1991.tb01481.x
Subject(s) - glycolysis , oxidative phosphorylation , catecholamine , medicine , endocrinology , receptor , stimulation , chemistry , water transport , phosphorylation , glucose transporter , biology , biophysics , metabolism , biochemistry , water flow , environmental engineering , insulin , engineering
SUMMARY 1. Catecholamine‐induced water transport was measured using an everted gut sac technique. Adrenaline, noradrenaline and isoprenaline induce dose‐dependent increases in water transport by the proximal intestinal sacs. Use of selective adrenergic agents revealed the possible involvement of α 1 ‐ and β 2 ‐receptors in mediation of catecholamine stimulation of water transport in this segment. 2. Inhibition of glycolysis reduced the effect mediated through α 1 ‐receptors, while the inhibition of oxidative phosphorylation blocked the β 2 ‐receptor mediated increase in water transport. 3. Basal transport of water was also significantly reduced by inhibition of glycolysis but was significantly elevated by blockage of oxidative phosphorylation. 4. Suppression or stimulation of glycolysis was paralleled by similar changes in lactic acid release from the gut wall. 5. It is concluded that the energy for the catecholamine‐induced water transport is contributed by glycolysis and oxidative phosphorylation coupled to α 1 ‐ and β 2 ‐receptors, respectively. Under basal conditions water transport is mainly dependent on glycolysis in the segment of intestine examined.