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ANGIOTENSIN‐CONVERTING ENZYME IN MACRO‐AND MICROVESSELS OF THE RAT
Author(s) -
Jandeleit Karin,
Jackson Bruce,
Perich Rose,
Paxton Donna,
Johnston Colin
Publication year - 1991
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1991.tb01462.x
Subject(s) - dissociation constant , angiotensin converting enzyme , in vivo , radioligand , endocrinology , medicine , aorta , mesenteric arteries , chemistry , enzyme , blood vessel , angiotensin ii , receptor , biochemistry , biology , blood pressure , artery , microbiology and biotechnology
SUMMARY 1. Angiotensin‐converting enzyme (ACE) was measured in the aorta, cerebral and mesenteric microvessels of Wistar‐Kyoto rats using a specific radioligand assay. 2. Ligand binding studies revealed a four‐fold increase in concentration of ACE in mesenteric microvessels, compared with aorta or brain microvessels. The dissociation constant ( K d ) was similar in all tissues. 3. The effects of oral administration of the ACE inhibitor S9650 on vascular ACE in vivo were measured. 4. S9650 produced a dose‐dependent inhibition of vascular ACE from brain and mesenteric microvessels.