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CULTURING RAT NEONATAL MYOCYTES CAUSES CHANGES IN THE PHOSPHATIDYLINOSITOL TURNOVER PATHWAY
Author(s) -
Woodcock Elizabeth A.,
Fullerton Meryl,
Land Sarah,
Kuraja Ivana J.
Publication year - 1991
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1991.tb01457.x
Subject(s) - phosphatidylinositol , myocyte , microbiology and biotechnology , chemistry , medicine , endocrinology , biology , signal transduction
SUMMARY 1. Cultured neonatal myocytes are commonly used as a model system for the study of cardiac phosphatidylinositol (PI) turnover. 2. In neonatal myocytes stimulation with noradrenaline causes the release of the Ca 2+ ‐releasing compound inositol‐1,4,5‐ tris phosphate and the generation of the Ca 2+ ‐regulatory compound inositol‐1,3,4,5‐ tetrakis phosphate. 3. Addition of noradrenaline to intact, neonatal rat hearts stimulates the release of inositol‐1,4,5‐ tris phosphate, but not inositol‐1,3,4,5‐ tetrakis phosphate. 4. These findings show that the isolation and culture of the neonatal myocyte causes changes in the PI turnover pathway so that it becomes similar to that described in other cell types and different from that in intact myocardial tissue. 5. The neonatal myocyte is not a useful model for the study of cardiac PI turnover.