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HISTAMINE‐INDUCED CALCIUM OSCILLATIONS IN HUMAN VASCULAR SMOOTH MUSCLE: TEMPORAL SEQUENCE AND SPATIAL ORGANIZATION IN SINGLE CELLS
Author(s) -
Neylon Craig B.,
Mason William T.,
Irvine Robin F.
Publication year - 1991
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1991.tb01450.x
Subject(s) - population , histamine , calcium , oscillation (cell signaling) , vascular smooth muscle , chemistry , biophysics , biology , smooth muscle , neuroscience , anatomy , medicine , endocrinology , biochemistry , environmental health , organic chemistry
SUMMARY 1. Video imaging of single, fura2‐loaded vascular smooth muscle cells was used to examine the spatial and temporal alterations in calcium, Ca 2+ , in response to low levels of vasoconstrictor stimuli. 2. Histamine (0.5 μmol/L) produced repetitive oscillations in Ca 2+ , which appeared to show some variation in amplitude and frequency between cells. 3. Individual oscillations consisted of an initial increase in Ca 2+ in a localized region followed by a wave‐like propagation of this region of elevated Ca 2+ throughout the rest of the cell cytoplasm. 4. It is suggested that the subcellular spatial organization of Ca 2+ that was observed during a Ca 2+ oscillation allows a population of cells to operate in unison. Thus, oscillatory fluctuations in Ca 2+ may contribute to myogenic tone.