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NEUROPEPTIDE Y RADIO‐IMMUNOASSAY: CHARACTERIZATION AND APPLICATION
Author(s) -
Allen Roberta,
Boublik Jaroslav,
Hauger Richard L.,
Scott Neal,
Rivier Jean,
Brown Marvin R.
Publication year - 1991
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1991.tb01402.x
Subject(s) - immunoassay , characterization (materials science) , neuropeptide , medicine , nanotechnology , antibody , immunology , materials science , receptor
SUMMARY 1. A sensitive and specific neuropeptide Y (NPY) radio‐immunoassay has been developed. This radio‐immunoassay does not detect the NPY‐related peptides pancreatic polypeptide or peptide YY. NPY extracted from rat plasma using sequential C 18 sorbent and affinity chromatography co‐eluted with synthetic rat NPY when applied to high pressure liquid chromatography. 2. The procedure of stabilization of platelets followed by high speed centrifugation reduced basal values of NPY by 60%, and this may be consistent with removal of platelets that release NPY. Administration of the cholinesterase inhibitor physostigmine (0.3 mg/kg), intravenously, produced a small but significant increase (39%) from basal concentrations of NPY. 3. NPY concentrations in young (2‐3‐month‐old) Sprague‐Dawley and Fisher 944 rats were similar; however, NPY concentrations were significantly increased (55%) in 2‐year‐old Fisher 944 rats. Similar to plasma concentrations of noradrenaline, NPY levels increase with age.