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TIME COURSE OF RECOVERY OF β‐ AND α 1 ‐ADRENOCEPTORS IN EXPERIMENTAL ASTHMA
Author(s) -
Shindoh Joe,
Sugiyama Satoru,
Hayashi Kouichi,
Takagi Kenzo,
Satake Tatsuo,
Ozawa Takayuki
Publication year - 1990
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1990.tb01348.x
Subject(s) - adenylate kinase , cyclase , endocrinology , prednisolone , medicine , adrenergic receptor , inhalation , adrenergic , guinea pig , chemistry , ovalbumin , asthma , receptor , anesthesia , immunology , immune system
SUMMARY 1. The time course of recovery of reduced β‐adrenoceptors caused by ovalbumin (OA) challenge was investigated using guinea‐pigs. 2. The effects of prednisolone on the recovery time course were also evaluated. 3. β‐ and α 1 ‐receptor assays were performed using lung membranes. Adenylate cyclase activity was also measured. 4. OA challenge reduced the number of β‐adrenoceptors by 35%, and a significant decrease (13%) persisted for 7 days. The number of β‐adrenoceptors recovered after 14 days. 5. OA challenge elevated the number of α 1 ‐adrenoceptors. A significant increase (24%) was observed after 7 days, and it took a further 7 days for the recovery. 6. After OA challenge there was a significant decrease in adenylate cyclase activity after 7 days, which recovered after a further 7 days. 7. Inhalation of prednisolone accelerated the recovery of β‐adrenergic responsiveness, though it did not affect the recovery of the number of α 1 ‐adrenoceptors. Prednisolone inhalation also elevated β‐adrenergic responsiveness in non‐asthmatic subjects. 8. It is concluded that reduced β‐adrenergic responsiveness caused by OA challenge persisted for 7 days and recovered after a further 7 days. Steroid hormone increased β‐adrenoceptors.