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DOES VERAPAMIL LIMIT MYOCARDIAL INFARCT SIZE IN A HEART DEFICIENT IN XANTHINE OXIDASE?
Author(s) -
Adachi Takeo,
Miura Tetsuji,
Noto Tetsuya,
Ooiwa Hitoshi,
Ogawa Takashi,
Tsuchida Akihito,
Iwamoto Toshihiro,
Goto Mahiko,
Iimura Osamu
Publication year - 1990
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1990.tb01279.x
Subject(s) - verapamil , xanthine oxidase , medicine , ischemia , heart rate , blood pressure , cardiology , anesthesia , calcium , chemistry , biochemistry , enzyme
SUMMARY 1. The delay of ischaemic myocardial necrosis by verapamil has been reported in the dog heart, which contains a high level of xanthine oxidase, a potential source of cytotoxic free radicals. To test whether the retardation of ischaemic myocyte death by verapamil is not an isolated phenomenon in the xanthine oxidase rich heart, we assessed the effect of verapamil in the rabbit heart, which lacks xanthine oxidase. 2. Verapamil (200 μg/kg, i.v. bolus plus 40 μg/kg per min) was administered in a group of rabbits ( n = 5) to test the haemodynamic response to this agent. The heart rate, blood pressure and left ventricular dp/dt max were reduced by 11, 25 and 57%, respectively, and the plasma concentration of verapamil was maintained at 300–400 ng/mL during the infusion. 3. In other groups of rabbits, the effect of the same dosage of verapamil on the size of myocardial infarct after 20 or 30 min ischaemia and 72 h reperfusion was examined. The verapamil was administered for 45 min, starting 15 min prior to ischaemia. The percentage of area at risk infarcted (%I/AAR) was 15.2±3.9% in the 20 min ischaemia control group and 15.4±4.5% in the 20 min ischaemia verapamil group, 49.1±3.4% in the 30 min ischaemia control group and 41.2±3.3% in the 30 min ischaemia verapamil group. The %I/AAR was significantly smaller in the 20 min ischaemia control groups and 15.4±4.5% in the 20 min ischaemia there was no difference in %I/AAR between the control and verapamil treated animals in either the 20 or the 30 min ischaemia groups. 4. These results suggest that verapamil does not delay the transition from reversible to irreversible myocardial injury during coronary occlusion in the rabbit, which like the human, lacks myocardial xanthine oxidase.