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NEW TOOLS FOR THE LOCALIZATION OF SECOND MESSENGER SYSTEMS
Author(s) -
Summers Roger J.,
McMartin Lynne R.
Publication year - 1989
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1989.tb01604.x
Subject(s) - second messenger system , forskolin , messenger rna , endoplasmic reticulum , protein kinase a , adenylate kinase , cyclase , complementary dna , chemistry , receptor , biology , enzyme , biochemistry , microbiology and biotechnology , gene
SUMMARY 1. Probes available for the localization of components of second messenger systems include G‐protein oligonucleotides which have been used to produce cDNA probes to label G‐protein mRNA by in situ hybridization histochemistry. 2. Enzymes involved in second messenger responses have been labelled with [ 3 H]‐forskolin (Gs‐linked adenylate cyclase), [ 3 H]‐cAMP (cAMP‐dependent protein kinase) and [ 3 H]‐PDBu 2 (protein kinase C). 3. [ 3 H]‐Inositol 1,4,5 trisphosphate labels a site on sarcoplasmic reticulum believed to trigger Ca 2+ release. 4. These probes allow the comparison of location of receptors and second messengers and the examination of changes in second messenger systems with drug treatment and disease.