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ENDOTHELIN RECEPTORS IN RAT ADRENAL GLAND VISUALIZED BY QUANTITATIVE AUTORADIOGRAPHY
Author(s) -
Kohzuki Masahiro,
Johnston Colin I.,
Chai Siew Yeen,
Casley David J.,
Rogerson Fraser,
Mendelsohn Frederick A. O.
Publication year - 1989
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1989.tb01550.x
Subject(s) - zona glomerulosa , adrenal gland , endocrinology , medicine , endothelin receptor , adrenal medulla , aldosterone , endothelin 1 , receptor , zona fasciculata , steroid 11 beta hydroxylase , biology , adrenal cortex , chemistry , catecholamine , angiotensin ii , steroid , hormone
SUMMARY 1. The radioligand [ 125 I]‐endothelin was used to map receptors for endothelin in rat adrenal gland using in vitro autoradiography and computerized densitometry. 2. In the adrenal, a high density of binding was found in the adrenal medulla (binding affinity constant 0.18 ± 0.11 × 10 9 M −1 ) and zona glomerulosa (binding affinity constant 0.18 ± 0.07 × 10 9 M −1 ). Binding was low to undetectable in the zona fasciculata and zona reticularis. Unrelated peptides did not displace endothelin. 3. These results provide evidence of endothelin receptor distribution in adrenal gland and suggest that endothelin might exert multiple actions in the adrenal gland on catecholamine and aldosterone biosynthesis and secretion.