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AROMATIC l ‐AMINO ACID DECARBOXYLASE: HISTOCHEMICAL LOCALIZATION IN RAT KIDNEY AND LACK OF EFFECT OF DIETARY POTASSIUM OR SODIUM LOADING ON ENZYME DISTRIBUTION
Author(s) -
Adam William R.,
Culvenor Anne J.,
Hall John,
Jarrott Bevyn,
Wellard R. Mark
Publication year - 1986
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1986.tb00314.x
Subject(s) - aromatic l amino acid decarboxylase , kidney , staining , potassium , endocrinology , medicine , chemistry , sodium , immunoperoxidase , enzyme , enzyme assay , biochemistry , biology , antibody , genetics , organic chemistry , immunology , monoclonal antibody
SUMMARY 1. Utilizing a mono‐specific antiserum produced in rabbits to hog kidney aromatic l ‐amino acid decarboxylase (AADC), the enzyme was localized in rat kidney by immunoperoxidase staining. 2. AADC was located predominantly in the proximal convoluted tubules; there was also weak staining in the distal convoluted tubules and collecting ducts. 3. An increase in dietary potassium or sodium intake produced no change in density or distribution of AADC staining in kidney. 4. An assay of AADC enzyme activity showed no difference in cortex or medulla with chronic potassium loading. 5. A change in distribution or activity of renal AADC does not explain the postulated dopaminergic modulation of renal function that occurs with potassium or sodium loading.