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THE RELATIONSHIP BETWEEN TISSUE LEVELS OF CYCLIC GMP AND TRACHEAL SMOOTH MUSCLE RELAXATION IN THE GUINEA‐PIG
Author(s) -
Suzuki Kiyoshi,
Takagi Kenzo,
Satake Tatsuo,
Sugiyama Satoru,
Ozawa Takayuki
Publication year - 1986
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1986.tb00313.x
Subject(s) - nicorandil , sodium nitroprusside , smooth muscle , chemistry , muscle relaxation , guinea pig , acetylcholine , cyclic gmp , medicine , cyclic nucleotide , endocrinology , nitric oxide , biochemistry , nucleotide , enzyme , gene
SUMMARY 1. The effect of cyclic GMP was investigated using guinea‐pig tracheal smooth muscle. 2. 8‐Bromo‐cyclic GMP showed a dose‐dependent relaxation of spontaneous tension of tracheal smooth muscle. 3. Administration of sodium nitroprusside induced dose‐dependent relaxation of tracheal smooth muscle as well as an increase in tissue levels of cyclic GMP. 4. Nicorandil, N ‐(2‐hydroxyethyl) nicotinamide nitrate showed dose‐dependent relaxation of tracheal smooth muscle and an increase in cyclic GMP levels in the tissue. 5. N ‐(2‐aminoethyl) nicotinamide dihydrochloride, which is a nicorandil derivative and differs minimally in its molecular structure (‐NH 2 vs ‐NO 2 ), had neither a relaxant effect on tracheal smooth muscle nor did it increase the level of cyclic GMP in the tissue. 6. The rise in cyclic GMP levels preceded the relaxation of tracheal smooth muscle induced by sodium nitroprusside. 7. These results suggest that cyclic GMP is one of the relaxant factors and that nitro‐derivatives exhibit their relaxant effect on the smooth muscle mediated by an increase in cyclic GMP level.