Premium
FURTHER STUDIES ON THE EFFECTS OF ADRENAL STEROIDS IN THE ACTIVE TRANSPORT OF SEROTONIN INTO RAT PLATELETS
Author(s) -
Lee P. H. K.,
Chan MoYin
Publication year - 1985
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1985.tb00301.x
Subject(s) - endocrinology , medicine , serotonin , platelet , corticosterone , chemistry , glucocorticoid , dexamethasone , potency , in vitro , hormone , biochemistry , receptor
SUMMARY 1. Male rats were treated with a fixed dose of aminoglutethimide (50 mg/kg s.c.) or with progressively increasing doses (50‐100 mg/kg s.c.) for 3 days. Corticosterone levels were found to be decreased in the latter group. Platelet uptake of serotonin as well as the apparent V max were decreased, whereas the K m of uptake were increased when compared with that of the control group. 2. Addition of ACTH (10 iu/dl) to control rat platelet and corticosterone (10‐80 μg/dl) or triamcinolone (0.5‐5.0 μg/dl) to adrenalectomized rat platelet suspension in vitro did not increase the serotonin uptake of the preparation. 3. Administration of exogenous dexamethasone (0.05‐0.2 mg/kg i.m.) or triamcinolone (0.05‐5.0 mg/kg i.m.) to adrenalectomized rats, caused a dose related increase in active uptake of serotonin by the platelets. Deoxycorticosterone (0.1‐1.0 mg/kg i.m.) did not have this effect. 4. The time course of response to, and the maximum percentage increase in platelet serotonin uptake by, exogenous corticosteroids are related to their glucocorticoid potency. 5. The possible role of glucocorticoids on platelet serotonin uptake process is discussed.