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THE MEASUREMENT OF PLASMA THROMBOXANE B 2 AND THE EFFECT OF SMOKING
Author(s) -
Sturm M.,
Barden A.,
Beilin L. J.,
Taylor R. R.
Publication year - 1984
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1984.tb00874.x
Subject(s) - thromboxane b2 , radioimmunoassay , chemistry , venous blood , thromboxane , blood sampling , sampling time , medicine , platelet , chromatography , endocrinology , biochemistry , statistics , mathematics
SUMMARY 1. Plasma thromboxane B 2 (TxB 2 ) was measured by radioimmunoassay using an iodinated ligand following extraction and further purification by thin layer chromatography. Venous blood was sampled into a syringe containing the cyclooxygenase inhibitor meclofenamate. 2. Normal levels, 15 pg/ml (s.d. = 8, n = 21), were lower than usually reported and measured values increased several fold over 20 min sampling from an indwelling needle. 3. With appropriate sampling there was a statistically insignificant increase in plasma TxB 2 after subjects smoked two cigarettes ( n = 11). An average decrease occurred in a control group ( n = 10) and the difference between groups was of borderline significance ( P <0.05). Smoking did not change TxB 2 production associated with platelet aggregation induced in vitro by collagen, whereas plasma adrenaline increased significantly. 4. The results emphasize the importance of the technique of sampling and assay in the measurement of plasma TxB 2 .