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AN IMPORTANT EFFECT OF TSH ON PROSTACYCLIN CONCENTRATION IN PORCINE THYROID CELLS
Author(s) -
Takasu N.,
Takahashi K.,
Ishigami T.,
Shimizu Y.,
Yamada T.,
Sato S.
Publication year - 1984
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1984.tb00256.x
Subject(s) - endocrinology , medicine , prostacyclin , thyroid , prostaglandin , metabolite , chemistry , hormone , prostaglandin e
SUMMARY 1. Fresh thyroid slices obtained from normal pigs contained prostaglandin E 2 (PGE 2 ), prostaglandin F 2α (PGF 2α ) and prostacyclin (PGI 2 , measured as the end metabolite, 6‐keto PGF 1α ), but the concentrations of PGI 2 exceeded those of PGE 2 and PGF 2α . A preferential increase of PGI 2 was produced when the slices were exposed to a large dose of TSH (thyroid‐stimulating hormone). 2. When thyroid cells were isolated and cultured in the absence of TSH, thyroidal concentrations of PGI 2 , PGE 2 and PGF 2α were about equal. When the cells were acutely exposed to a large dose of TSH, intrathyroidal concentration of prostaglandin increased significantly, but the relative concentrations of prostaglandins were not markedly altered. 3. When thyroid cells were cultured in the presence of a small dose of TSH, relative concentrations of thyroidal prostaglandins were comparable to those of fresh thyroid slices, as a result of a preferential increase of PGI 2 and decreases of PGE 2 and PGF 2α . Further increase of PGI 2 was induced when the cells cultured with TSH were acutely exposed to a large dose of TSH. 4. It is concluded that although cells contain PGE 2 , PGF 2α and PGI 2 , a preferential accumulation of PGI 2 is found only when thyroid cells are chronically stimulated by TSH.