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EFFECTS OF ADRENALECTOMY AND ADRENAL STEROIDS ON THE UPTAKE OF SEROTONIN IN RAT PLATELETS
Author(s) -
Lee P. H. K.,
Chan MoYin
Publication year - 1984
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1984.tb00239.x
Subject(s) - triamcinolone acetonide , serotonin , endocrinology , adrenalectomy , medicine , platelet , chemistry , in vivo , ex vivo , in vitro , biology , biochemistry , surgery , receptor , microbiology and biotechnology
SUMMARY 1. Changes induced by adrenalectomy, triamcinolone treatment and deoxycorticosterone (DOC) treatment on the uptake of [ 3 H]‐serotonin into platelets were studied ex vivo , using rat platelets suspended in physiological medium. 2. Adrenalectomy caused a decrease in active uptake of serotonin and an associated increase in apparent K m . 3. Treatment of the adrenalectomized rats with triamcinolone (0.25 mg/kg), but not DOC (1.0 mg/kg) restored the active uptake of serotonin. 4. At triamcinolone dose of 0.5 mg/kg, the serotonin uptake was raised to a level that was significantly higher than normal. 5. Kinetic analysis of uptake data showed the apparent K m , to be affected by both triamcinolone and DOC, while the apparent V max of uptake rate was increased by triamcinolone treatment.