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THE MECHANISM OF POLYURIA IN RATS PRETREATED WITH LITHIUM STUDIES BY IN VITRO MICROPERFUSION
Author(s) -
Ray C.,
Morgan T.,
Carney S.
Publication year - 1983
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1983.tb00181.x
Subject(s) - chemistry , medicine , endocrinology , lithium (medication) , polyuria , antidiuretic , in vitro , permeability (electromagnetism) , hormone , membrane , biochemistry , biology , diabetes mellitus
SUMMARY The collecting ducts in papillae taken from normal rats have a measurable increase in diffusional tritiated water (THO) permeability with ADH 5 μunit/ml and this increase is maximal with antidiuretic hormone (ADH) 100 μunit/ml added to the media. 2 The presence of plasma from rats pretreated with lithium to make them polyuric inhibited the response to ADH. The lowest concentration of ADH that caused a measurable increase in diffusional water permeability was 50 μunit/ml and the increase was maximal with ADH 2000 μunit/ml. 3 The maximum response to ADH did not differ whether plasma from control or lithium pretreated rats was used. However, the dose‐response curve to ADH was shifted to the right by the plasma from lithium‐pretreated rats. 4 Lithium added to the plasma from control rats did not alter the response to ADH. 5 It is proposed that lithium given to rats causes a circulatory factor to be produced that inhibits in a competitive fashion the response of the collecting duct to ADH. Such an effect would explain many features of the impairment of water excretion associated with lithium use.