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ESTIMATION OF LECITHIN IN RABBIT TISSUES
Author(s) -
Gardiner J.E.,
Lee H.S.
Publication year - 1982
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1982.tb00778.x
Subject(s) - lecithin , chloroform , chromatography , chemistry , reagent , methanol , acetic acid , solvent , kidney , rabbit (cipher) , biochemistry , biology , endocrinology , organic chemistry , statistics , mathematics
SUMMARY 1. A simple method for lecithin estimation was developed. Phospholipids in rabbit tissues were extracted with chloroform and methanol. 2. The samples were chromatographed with eluting solvent system; chloroform:methanol:glacial acetic acid:water (100:25:8:1.1). Lecithin was identified by the spray reagent as blue spots and the intensities were quantitated with a den sitometer. 3. The concentration of lecithin in the various rabbit tissues varied from 2.8 to 10 mg/g tissue. The sequence of increasing lecithin concentration was heart < kidney < lung < liver < brain. 4. Recovery experiments of added lecithin into the tissues showed a recovery of 91–104%.