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THE CONTRACTION INDUCED BY ETHYLENEDIAMINETETRACETIC ACID‐4 SODIUM (EDTA‐4Na) IN GUINEA‐PIG ISOLATED VAS DEFERENS KEPT IN ISOTONIC SUCROSE MEDIUM
Author(s) -
Sugimoto J.,
Furumichi A.,
Murakami M.,
Morita M.
Publication year - 1978
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1978.tb00701.x
Subject(s) - sucrose , contraction (grammar) , vas deferens , chemistry , sodium , muscle contraction , isotonic , biochemistry , biophysics , chromatography , medicine , endocrinology , biology , organic chemistry
SUMMARY 1. Ethylenediaminetetracetic acid‐4 sodium (EDTA–4Na) induced a contraction in guinea‐pig isolated vas deferens kept in isotonic sucrose medium containing 0.07%NaHCO 3 and 0.1% glucose after preincubation in Locke's solution. The maximum contraction was 82.1 ± 6.7% of that induced by added KCl. 2. EDTA‐Ca‐2Na (10 ‐7 to 10 ‐3 g/ml) induced no contraction in preparations. kept in the sucrose medium. 3. The EDTA‐4Na‐induced contraction was also obtained when sucrose in the sucrose medium was replaced by glucose but not when the preparation was kept in Locke's solution. 4. The EDTA‐4Na‐induced contraction was decreased by reduction of the CaCl 2 or KC1 concentration of the Locke's solution in which the preparation had been preincubated before placing in the sucrose medium. 5. The EDTA‐4Na‐induced contraction was suppressed with increasing concentrations of NaCl used to partially replace sucrose in the sucrose medium. 6. When the preparation was kept in EDTA‐free sucrose medium after pretreat‐ment with 10 ‐4 g/ml of EDTA‐4Na in the sucrose medium for 20 min, a contraction was induced by added CaCl 2 . The maximum contraction induced by CaCl 2 was 30.1 ± 11.8% of that of the EDTA‐4Na‐induced contraction, when CaCl 2 was added at 20 min after the exchange of medium.