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FORMATION OF A TERNARY COMPLEX WITH SERUM ALBUMIN: AN EXPLANATION FOR THE EFFECT OF α‐MERCAPTO‐β‐(2‐FURAN) ACRYLIC ACID ON RAT PLASMA ZINC CLEARANCE
Author(s) -
Giroux Eugene,
Prakash N. J.,
Schechter Paul J.
Publication year - 1977
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1977.tb02374.x
Subject(s) - zinc , chemistry , albumin , stoichiometry , acrylic acid , serum albumin , nuclear chemistry , mole , polymer chemistry , organic chemistry , biochemistry , polymer , monomer
SUMMARY 1. Four, six and eight hours after gavage of rats with α‐mercapto‐β‐(2‐furan) acrylic acid (MFA) (25 mg/kg) serum zinc concentration was increased ten‐fold over control levels and a mean molar ratio of 1 albumin:0.4 zinc:0.8 MFA was found for seventeen sera. 2. At pH 7.5 a maximum of 1 mole of MFA could be bound per mole of metal‐free bovine serum albumin. 3. In the presence of zinc ion, albumin‐zinc‐MFA complexes formed, since for each mole of albumin‐zinc complex an additional mole of MFA could be bound to albumin. Complexes up to a molar stoichiometry of 1 albumin:2 zinc:3 MFA were prepared. 4. MFA stabilized the albumin‐zinc complex against dissociation. 5. Formation of similar complexes in vivo may account for the markedly delayed clearance of plasma zinc seen in rats administered β‐aryl derivatives of α‐mercaptoacrylic acid.