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MINERALOCORTICOID RECEPTORS IN SHEEP KIDNEY AND PAROTID STUDIES IN NA+ REPLETE AND NA+ DEPLETE STATES
Author(s) -
Butkus Aldona,
Coghlan J. P.,
Paterson R. A.,
Scoggins B. A.,
Robinson Julie A.,
Funder J. W.
Publication year - 1976
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1976.tb00637.x
Subject(s) - mineralocorticoid , endocrinology , mineralocorticoid receptor , receptor , kidney , medicine , chemistry
SUMMARY 1. As an adrenalectomized sheep becomes progressively more Na + deplete, its salivary electrolyte secretion becomes increasingly sensitive to exogenous aldosterone administration. 2. Alteration in mineralocorticoid receptor affinity or concentration is a possible mechanism whereby this sensitivity could be expressed at a molecular level. 3. Binding sites with a high affinity for aldosterone (K d 37°C∼5 × 10 ‐10 M) have been demonstrated in sheep kidney and parotid. 4. Renal binding sites were examined in detail, and shown to have affinity characteristics appropriate for mineralocorticoid receptors (aldosterone>deoxycorticosterone>dexamethasone>cortisol>progesterone>oestradiol > dihydrotestosterone). 5. No difference in mineralocorticoid receptor affinity or concentration could be shown between Na + replete and Na + deplete sheep, in either kidney or parotid gland. 6. Accordingly, the molecular mechanisms whereby parotid sensitivity is mediated remain as yet undefined.