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THE INTERRELATIONSHIPS BETWEEN ANTIDIURETIC HORMONE, ADENYL CYCLASE, TISSUE CYCLIC AMP AND DIFFUSIONAL WATER PERMEABILITY
Author(s) -
Rayson B. M. R.,
Carney S.,
Gardner G.,
Morgan T.
Publication year - 1976
Publication title -
clinical and experimental pharmacology and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 103
eISSN - 1440-1681
pISSN - 0305-1870
DOI - 10.1111/j.1440-1681.1976.tb00599.x
Subject(s) - theophylline , cyclase , antidiuretic , medicine , permeability (electromagnetism) , endocrinology , chemistry , adenylate kinase , hormone , kidney , second messenger system , biology , membrane , biochemistry , enzyme
SUMMARY 1. Physiological concentrations of antidiuretic hormone increase diffusional water permeability but not measurable cyclic AMP content in the isolated papilla of the rat's kidney. 2. Theophylline (6 MM) increases diffusional water permeability and cyclic AMP content in the isolated papilla of the rat's kidney. 3. The increase in water permeability is detected with 5 μunits. ml ‐1 of ADH and is maximal with 50 μunits. ml ‐1 . The same maximum was achieved with 6 MM theophylline. 4. Cyclic AMP and dibutyryl cyclic AMP both increase water permeability, but to a lesser extent than theophylline or ADH. 5. In the presence of theophylline, ADH causes a dose related generation of tissue cyclic AMP up to a dose of 2,000,000 μunits. ml ‐1 . 6. Adenyl cyclase is increasingly activated by ADH up to doses of 2,000,000 μunits. ml ‐1 . 7. These results suggest that while ADH activates the adenyl cyclase system and changes water permeability there are sufficient disparities to cast doubt on an exclusive role for cyclic AMP as the second messenger.