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Effect of HSPC016 gene expression on the aggregative growth of dermal papillae cells
Author(s) -
Song Zhiqiang,
Zhou Chunli,
Wang Jiwen,
Yang Weibin,
Hao Fei
Publication year - 2012
Publication title -
australasian journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.67
H-Index - 53
eISSN - 1440-0960
pISSN - 0004-8380
DOI - 10.1111/j.1440-0960.2010.00700.x
Subject(s) - transfection , pichia pastoris , recombinant dna , small interfering rna , dermal papillae , gene expression , gene , microbiology and biotechnology , rna interference , rna , biology , genetics , hair follicle
ABSTRACT In previous studies, HSPC016 was identified as a differentially expressed gene in dermal papilla cells (DPC) with aggregative behaviour. To clarify its role in the regulation of DPC, the recombinant HSPC016 protein was expressed using the Pichia pastoris expression system, and three small interfering ribonucleic acid duplexes (siRNA) were designed and transfected into DPC. Results showed that DPC with the HSPC016 gene inhibited, exhibit non‐aggregative behaviour and grow much slower than normal DPC, and that the recombinant HSPC016 protein could promote the proliferation of high‐passage DPC and induce its aggregative behaviour.