Premium
IN VITRO AND IN VIVO METHODS TO DEFINE SUNSCREEN PROTECTION
Author(s) -
Groves Gordon A.,
Agin Patricia Poh,
Sayre Robert M.
Publication year - 1979
Publication title -
australasian journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.67
H-Index - 53
eISSN - 1440-0960
pISSN - 0004-8380
DOI - 10.1111/j.1440-0960.1979.tb00217.x
Subject(s) - hairless , in vitro , in vivo , medicine , erythema , epidermis (zoology) , human skin , dermatology , pharmacology , biology , anatomy , biochemistry , microbiology and biotechnology , genetics
S ummary The potential ability of nine sunscreen preparations to prevent erythema was assessed by two commonly used in vitro methods—the thin‐film technique and the dilute‐solution method—as well as by a new in vitro method—the hairless mouse procedure. The study indicates that there are serious deficiencies in these first two methods when their results are compared to those obtained from in vivo testing. These two spectrophotometric methods predicted a much higher decree of effectiveness than was obtained under in‐use conditions. The new in vitro method described here that uses the excised epidermis of the hairless mouse gave results comparable to those obtained in human volunteers.