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Somatic Cell Nuclear Transfer and Transgenesis in Large Animals: Current and Future Insights
Author(s) -
Galli C,
Lagutina I,
Perota A,
Colleoni S,
Duchi R,
Lucchini F,
Lazzari G
Publication year - 2012
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2012.02045.x
Subject(s) - somatic cell nuclear transfer , transgenesis , reprogramming , transgene , biology , somatic cell , genome , genome editing , livestock , regenerative medicine , computational biology , genetics , microbiology and biotechnology , genetically modified organism , stem cell , embryo , cell , gene , reproductive technology , embryogenesis , blastocyst , ecology
Contents Somatic cell nuclear transfer (SCNT) was first developed in livestock for the purpose of accelerating the widespread use of superior genotypes. Although many problems still exist now after fifteen years of research owing to the limited understanding of genome reprogramming, SCNT has provided a powerful tool to make copies of selected individuals in different species, to study genome pluripotency and differentiation, opening new avenues of research in regenerative medicine and representing the main route for making transgenic livestock. Besides well‐established methods to deliver transgenes, recent development in enzymatic engineering to edit the genome provides more precise and reproducible tools to target‐specific genomic loci especially for producing knockout animals. The interest in generating transgenic livestock lies in the agricultural and biomedical areas and it is, in most cases, at the stage of research and development, with few exceptions that are making the way into practical applications.