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Effect of Freezing on Sperm Nuclear DNA
Author(s) -
Fraser L,
Strzeżek J,
Kordan W
Publication year - 2011
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2011.01815.x
Subject(s) - dna fragmentation , sperm , andrology , dna damage , semen , chromatin , nuclear dna , sperm quality , dna , biology , boar , microbiology and biotechnology , genetics , mitochondrial dna , apoptosis , medicine , gene , programmed cell death
Contents Sperm DNA damage has a significant impact on reproductive outcomes. In recent years, the search for optimal molecular markers for the evaluation of semen quality has resulted in the increased focus on sperm nuclear DNA assessment. The primary aim of this article was to review and summarize the effects of freezing–thawing procedure on nuclear DNA integrity of boar spermatozoa. Using different sperm DNA integrity assays, it has been confirmed that the sperm DNA undergoes structural changes during the freezing–thawing process. Evidence has been shown that a significant proportion of frozen‐thawed spermatozoa with compromised chromatin integrity was highly susceptible to DNA fragmentation. Moreover, the possible mechanisms responsible for post‐thaw sperm DNA damage could be because of cryo‐induced oxidative stress and, to a lesser extent, to the activation of an apoptotic‐like phenomenon. This review also highlights the ongoing effort employed to develop optimal strategies to reduce sperm DNA damage following freezing–thawing of boar semen.