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Effects of Activation Methods on DNA Synthesis and Development of Parthenogenetic Porcine Embryos
Author(s) -
Ock SA,
Kwack DO,
Mohana Kumar B,
Han J,
Kim SW,
Rho GJ
Publication year - 2011
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2011.01790.x
Subject(s) - parthenogenesis , embryo , dna synthesis , andrology , dna , biology , microbiology and biotechnology , chemistry , genetics , medicine
Contents This study investigated the timing of DNA synthesis and patterns of pronuclear (PN) formation during the first cell cycle, and its influence on developmental competence, velocity and proliferation index of porcine parthenote blastocysts produced by different activation treatments. Oocytes were activated as follows: electrical stimulation (EST), EST combined with 7.5 μg/ml cytochalasin B (EST + CCB), 10 μg/ml cycloheximide (EST + CHX) and 1.9 m m 6‐dimethylaminopurine (EST + 6‐DMAP) for 3 h. DNA synthesis and PN formation were evaluated using 1 m m 5′bromo‐2′deoxy‐uridne (BrdU) at 2 h intervals from 1 to 13 h or 5 to 13 h of post‐activation (hpa), respectively. In EST, DNA synthesis started at 3 hpa, reached the peak at 11 hpa and decreased at 13 hpa. Treatment with 6‐DMAP resulted in an early increase of DNA synthesis at 3 hpa, whereas CCB delayed DNA synthesis for 2 h. In EST and EST + 6‐DMAP, most of the eggs showed 1PN, whereas, incidence of 2PN in EST + CCB was higher than 1PN. EST + CHX was observed with 1PN, 2PN and multiple PN. Blastocyst rate in EST + CCB and EST + 6‐DMAP were significantly (p < 0.05) higher than EST + CHX. But, the developmental velocity was not different among groups. Proliferation index of blastocysts, as indicated the number of blastomere at S‐phase of the cell cycle was low in all groups. In conclusion, CCB, CHX and 6‐DMAP used for producing porcine parthenogenetic embryos induced different onset of DNA synthesis and PN, but they did not affect the subsequent embryo development.