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Extenders Containing Dimethylformamide Associated or Not with Glycerol are Ineffective for Ovine Sperm Cryopreservation
Author(s) -
Moustacas VS,
Cruz BC,
Varago FC,
Miranda DA,
Lage PG,
Henry M
Publication year - 2011
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2011.01785.x
Subject(s) - extender , glycerol , cryoprotectant , cryopreservation , semen cryopreservation , dimethylformamide , sperm , semen , chemistry , sperm motility , andrology , biology , biochemistry , embryo , medicine , organic chemistry , solvent , microbiology and biotechnology , polyurethane
Contents The study aimed at testing the effectiveness of dimethylformamide, alone or combined with glycerol, as cryoprotectant for freezing ram semen. Ejaculates from nine rams were cryopreserved in Tris‐based extenders, containing 5% of glycerol, association of dimethylformamide with glycerol, in four proportions achieving 5% of cryoprotectors in the media and pure dimethylformamide (2, 3, 4 and 5%) in replacement to glycerol. The samples were diluted to 100 × 10 6 sptz/ml and stored in 0.25‐ml straws in liquid nitrogen. After thawing (37°C for 30 s), motility was preserved better by the extender containing 5% of glycerol (p < 0.05). The extenders containing pure dimethylformamide, or more than 2% in combination with glycerol, provided sperm motilities close to zero. Plasma and acrosomal membrane integrity were preserved better (p < 0.05) in the extender containing 5% glycerol. It can be concluded that dimethylformamide, alone or combined with glycerol, has no beneficial effects on ovine semen cryopreservation.