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Expression of Pluripotency Genes in Buffalo ( Bubalus bubalis ) Amniotic Fluid Cells
Author(s) -
Yadav PS,
Mann A,
Singh V,
Yashveer S,
Sharma RK,
Singh I
Publication year - 2011
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2010.01733.x
Subject(s) - homeobox protein nanog , biology , bubalus , amniotic fluid , andrology , microbiology and biotechnology , stem cell , multipotent stem cell , embryonic stem cell , gene , progenitor cell , fetus , pregnancy , genetics , induced pluripotent stem cell , medicine , ecology
Contents Recent findings suggest that mammalian amniotic fluid (AF) is a source of multipotent stem cells (SCs), which can be used in regenerative medicine and assisted reproduction. We report the isolation, culture and characterization of amniotic fluid‐derived cells from pregnant water buffalo uterus. These undifferentiated AF cells expanded without feeder layer over a period of 100 days up to passages 20 and the expression of alkaline phosphatase (AP), Oct‐4, Nanog and Sox‐2, GAPDH and β‐actin could be detected by RT‐PCR. The cells exhibited uniform morphology and normal chromosome number. The up‐regulation or down‐regulation of transcription factors of each gene varied with passage number. We conclude that putative bubaline AF cells can be cultured and maintained in vitro for a prolonged period and offer a potential source of multipotent cells for applications including assisted reproduction in buffalo.