Premium
Improvement of 2D‐PAGE Resolution of Human, Porcine and Equine Follicular Fluid by Means of Hexapeptide Ligand Library
Author(s) -
Fahiminiya S,
Labas V,
Dacheux JL,
Gérard N
Publication year - 2011
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2010.01683.x
Subject(s) - follicular fluid , follicular phase , ligand (biochemistry) , abundance (ecology) , high resolution , follicle , chemistry , biology , microbiology and biotechnology , receptor , biochemistry , endocrinology , oocyte , embryo , remote sensing , fishery , geology
Contents The major challenge of follicular fluid proteomic analysis is the presence of high‐abundance proteins that originate from plasma. These proteins can prevent the detection of lower abundant ones, produced locally by follicle cells and that may have important roles in follicular activity. In this study, the novel technology called hexapeptide ligand library was evaluated to enrich the low‐abundance proteins in follicular fluid of human (HFF), porcine (PFF) and equine (EFF) prior 2D‐PAGE. Our results showed that the new strategy enabled detection of many new protein spots, increased resolution and highly improved the intensity of low‐abundance proteins by 2D‐PAGE.