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Single Layer Centrifugation of Stallion Spermatozoa through Androcoll™‐E does not Adversely Affect their Capacitation‐Like Status, as Measured by CTC Staining
Author(s) -
Bergqvist AS,
Johannisson A,
Bäckgren L,
Dalin AM,
RodriguezMartinez H,
Morrell JM
Publication year - 2011
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2010.01637.x
Subject(s) - capacitation , andrology , sperm , acrosome reaction , staining , acrosome , centrifugation , semen , sperm motility , motility , biology , chemistry , biochemistry , microbiology and biotechnology , medicine , genetics
Contents This study was designed to evaluate the effect of single layer centrifugation (SLC) and subsequent cold storage on stallion sperm capacitation‐like status and acrosome reaction. Three stallions were included in the study, with three ejaculates per stallion. The samples were examined 4, 24 and 72 h after collection, extension and SLC, with storage at 6°C. Sperm capacitation‐like status was investigated using the fluorescent dye chlortetracycline (CTC). There was no difference in capacitation‐like status between colloid‐selected and non‐selected spermatozoa. Sperm motility decreased significantly during cold storage, whereas the proportion of apparently capacitated spermatozoa increased. There was no change in the proportion of acrosome‐reacted spermatozoa. In conclusion, SLC through Androcoll™‐E does not adversely affect the capacitation‐like status of stallion spermatozoa, although it did increase with time during cold storage.