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Effects of Different Oocyte Activation Procedures on Development and Gene Expression of Porcine Pre‐Implantation Embryos
Author(s) -
Cervera RP,
Silvestre MA,
Martí N,
GarcíaMengual E,
Moreno R,
Stojkovic M
Publication year - 2010
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2009.01509.x
Subject(s) - somatic cell nuclear transfer , oocyte activation , oocyte , ionomycin , embryo , andrology , gene expression , parthenogenesis , blastocyst , embryo transfer , biology , transcriptome , primer (cosmetics) , microbiology and biotechnology , gene , chemistry , embryogenesis , genetics , medicine , intracellular , organic chemistry
Contents Among the factors that affect the efficiency of somatic cell nuclear transfer (SCNT) in pigs, the activation protocol is the most variable among the current SCNT procedures. The aim of this study is focused on defining an efficient activation treatment of porcine oocytes. In Experiment 1, we studied the effects of nine different oocyte activation procedures (including chemical‐ and electrical‐based treatments) on parthenogenetic embryo development. In Experiment 2, we studied the effect of the more efficient activation procedures on the gene expression profile of Oct4 and Igf2r in parthenogenetic blastocysts. In conclusion, ionomycin as a first calcium stimulus is not able to activate porcine oocytes efficiently in comparison with electric procedures. Electrical treatments with 6‐DMAP significantly increased the level of Oct4 expression, whereas the single and double pulse treatments alone maintained the same profile as the IVF group.