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Site of Intrauterine Artificial Insemination in the Bitch does not Affect Sperm Distribution within the Uterus
Author(s) -
Fukushima FB,
Malm C,
Henry M,
Gheller VA,
Serakides R,
Neves MM,
Macedo SP,
Figueiredo MS,
Andrade MEJ,
Chaves MS,
Silva MX,
Rezende CMF,
Melo EG
Publication year - 2010
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2009.01493.x
Subject(s) - uterine horns , uterus , insemination , oviduct , semen , artificial insemination , sperm , lumen (anatomy) , andrology , biology , anatomy , medicine , pregnancy , endocrinology , genetics , microbiology and biotechnology
Contents The aim of this study was to evaluate the distribution of frozen–thawed spermatozoa within the uterine lumen and oviducts following intrauterine laparoscopic deposition at two sites. Twelve bitches of unknown reproductive history were randomly distributed into two groups. Semen (3 ml containing 300 × 10 6 frozen–thawed spermatozoa) was infused at the uterine body (UB group) or at the cranial tip of the left uterine horn. A 22‐G catheter was used to access the uterine lumen. Sperm cell distribution was evaluated after ovariohysterectomy performed 3 h after artificial insemination (AI). There was no difference between groups in mean time to perform AI. Spermatozoa were detected in all uterine segments, including the tip of both horns, but none was detected in the oviduct. The 22‐G catheter facilitated deposition of semen in the uterine lumen, particularly at the UB site. Sperm cell distribution occurred evenly along both horns, independent of the site of semen deposition.