Premium
Parthenogenetic Activation of Pig Oocytes Using Pulsatile Treatment with a Nitric Oxide Donor
Author(s) -
Petr J,
Chmelíková E,
Krejčová T,
Řehák D,
Novotná B,
Jílek F
Publication year - 2010
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2008.01275.x
Subject(s) - nitric oxide , andrology , snap , blastocyst , oocyte , chemistry , parthenogenesis , pulsatile flow , embryo , medicine , endocrinology , embryogenesis , biology , microbiology and biotechnology , computer graphics (images) , computer science
Contents The nitric oxide donor (+)–S‐nitroso‐N‐acetylpenicillamine (SNAP) is capable of inducing parthenogenetic activation in pig oocytes matured in vitro . However, quite a long exposure to the nitric oxide donor, exceeding 10 h, is necessary for successful oocyte activation. Repeated short‐term treatment with 2 m m SNAP significantly increased the activation rates despite the fact that the overall exposure time to the nitric oxide donor did not exceed 4 h. With regard to the activation rate, 12 repeated treatments lasting 10 min each were found to be the most efficient regimen (63.3%). The continuous exposure to the nitric oxide donor for the same overall time induced parthenogenetic activation in 12.5% oocytes (2‐h continuous treatment with 2 m m SNAP). The development of parthenogenetic embryos increased after repeated short‐term treatment with SNAP. After continuous treatment with 2 m m SNAP for 10 h, only 6.7% of the oocytes cleaved, and none developed beyond the 4‐cell stage. Thirty‐minute treatment repeated four times with 2 m m SNAP induced cleavage in 37.5% of the oocytes, 18.3% developed to the morula stage, and 6.7% reached the blastocyst stage. Based on the results, it is concluded that pulsatile treatment can significantly improve parthenogenetic activation rate when compared with the continuous treatment using nitric oxide donors.