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The Effects of Sample Size on the Outcome of Ovarian Tissue Cryopreservation
Author(s) -
Ferreira M,
BosMikich A,
Frantz N,
Rodrigues JL,
Brunetto AL,
Schwartsmann G
Publication year - 2010
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2008.01261.x
Subject(s) - cryopreservation , follicular phase , ovarian tissue , histology , andrology , ovarian tissue cryopreservation , follicle , biology , ovarian follicle , ovary , endocrinology , medicine , embryo , fertility preservation , population , environmental health , microbiology and biotechnology , fertility , genetics
Contents Cryopreservation of ovarian tissue is known to affect follicular survival. Several variables may be responsible for this. Little attention has focused on the effect of the size of the fragment to be cryopreserved. This study was conducted to assess the effect of the size of the tissue on follicular histology after freezing with 1,2‐propanediol. Histological evaluations were performed of control and cryopreserved tissue. Fragments were cut 10 × 3 × 2 mm 3 (2 mm group) or 10 × 3 × 4 mm 3 (4 mm group). Percentages of normal follicles in control fragments cut into 2 and 4 mm slices were 56% and 34%, respectively. The relative risks to obtain normal follicles in the 2 mm and the 4 mm fragments after cryopreservation were 0.63 and 0.47, respectively. Freezing reduced follicle survival to a significantly greater extent in the larger tissue fragments. There is an increased risk of damage to primary and primordial follicles, when the tissue slices are cut with all dimensions larger than 2 mm.