Premium
Molecular Cloning and Characterization of CD9 cDNA from Sheep and Cashmere Goat
Author(s) -
Xing WJ,
Wang LQ,
Wu Q,
Ren SC,
Bao XH,
Bou S
Publication year - 2010
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2008.01195.x
Subject(s) - capra hircus , complementary dna , cashmere goat , biology , open reading frame , cloning (programming) , molecular cloning , ovis , transmembrane protein , transmembrane domain , rapid amplification of cdna ends , microbiology and biotechnology , gene , amino acid , peptide sequence , genetics , zoology , ecology , receptor , computer science , programming language
Contents CD9 is a glycoprotein of the transmembrane 4 superfamily (TM4SF) and is involved in various cellular processes. Some CD9 cDNA have been cloned in mammals and certain fish genera in recent years, but goat and sheep counterparts of cattle, human and mouse have not been identified. To facilitate the studies, we cloned the cDNA encoding for CD9 of cashmere goat ( Capra hircus ) and sheep ( Ovis aries ), and expressed sheep CD9 in Escherichia coli cells. Structural analysis indicated for both goat and sheep that a 1123 bp cDNA spanned an open reading frame of 681 bp which predicted a protein of 226 amino acids with a typical TM4SF structure, including four highly conserved transmembrane domains, two extracellular domains and a CCG motif, which is a hallmark of the TM4SF. The predicted amino acid sequences were highly homologous to those of cattle, mouse and human CD9. Molecular phylogenetic analysis based on CD9 cDNA sequences indicated that goat and sheep CD9 were closely related to CD9 of cattle, which is in agreement with their morphological taxonomy.