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Intracytoplasmic Sperm Injection in Livestock Species: An Update
Author(s) -
GarcíaRoselló E,
GarcíaMengual E,
Coy P,
Alfonso J,
Silvestre MA
Publication year - 2009
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2007.01018.x
Subject(s) - intracytoplasmic sperm injection , blastocyst , zygote , human fertilization , biology , in vitro fertilisation , reproduction , embryo , andrology , sperm , embryogenesis , ecology , agronomy , medicine , botany , microbiology and biotechnology
Contents Intracytoplasmic sperm injection (ICSI) is a powerful technique in the field of assisted reproduction (ART) and provides exciting opportunities for studying the basic mechanisms of fertilization and early embryo development. Nevertheless, its application in agriculture and conservation biology has been greatly hampered by the low success rate reported for this method in respect of economically important species. Specifically, the rates of blastocyst formation and live newborn are greatly reduced when zygotes are generated by ICSI. Except for humans, ICSI remains a low efficiency technology in comparison with alternatives such as in vitro fertilization (IVF) and its application is less widespread. In this paper, we discuss the present status, applications and factors affecting ICSI in pigs and other species.