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Expression of Interferon‐tau mRNA in Bovine Embryos Derived from Different Procedures
Author(s) -
Yao N,
Wan PC,
Hao ZD,
Gao FF,
Yang L,
Cui MS,
Wu Y,
Liu JH,
Liu S,
Chen H,
Zeng SM
Publication year - 2009
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2007.01009.x
Subject(s) - somatic cell nuclear transfer , blastocyst , conceptus , embryo , andrology , biology , messenger rna , interferon , microbiology and biotechnology , complementary dna , somatic cell , embryo transfer , in situ hybridization , gene expression , embryogenesis , immunology , fetus , gene , pregnancy , genetics , medicine
Contents Interferon‐tau (IFN‐τ) is a secreted conceptus protein which plays a critical role in the establishment of ruminant pregnancy by its antiluteolytic and antiviral effects. In the present study, we hypothesized that IFN‐τ expression was temporally and spatially regulated in different pre‐implantation embryos and the levels of IFN‐τ expression were different among bovine embryos derived from parthenogenetic activation (PA), in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT). By using in situ hybridization with Digoxingenin (DIG)‐labelled IFN‐τ cDNA as a probe, we detected IFN‐τ mRNA in bovine embryos from days 3 to 9 in culture. However, the timing of the initiation of IFN‐τ mRNA expression was different among PA, IVF and SCNT embryos. Interferon‐τ mRNA was first expressed in 16‐cell stage IVF embryos on day 4, in SCNT morula on day 5 and early PA blastocyst on day 6. Semi‐quantitative RT‐PCR analysis showed that the expression levels of IFN‐τ mRNA did not differ significantly among IVF, SCNT and PA embryos on day 7. In addition, freezing and thawing did not have a major impact either on IFN‐τ mRNA expression in IVF or in vivo ‐produced bovine blastocysts.