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Early Detection of Pregnancy and Embryonic Loss in Dairy Cattle by ELISA Tests
Author(s) -
Gábor G,
Tóth F,
Ózsvári L,
AbonyiTóth Zs,
Sasser RG
Publication year - 2007
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2006.00834.x
Subject(s) - cutoff , artificial insemination , zoology , insemination , pregnancy , dairy cattle , andrology , endocrinology , biology , medicine , sperm , genetics , physics , quantum mechanics
Contents Over a 25‐month period 8118 blood samples were assayed for the presence of the serum pregnancy specific‐protein B (PSPB) and progesteron (P4) concentrations on three Hungarian large‐scale dairy farms. Pregnancy (n = 4085) was checked by BioPRYN assay at 30–36 days post‐insemination (PI). Samples from all cows that tested not pregnant and from cows with an optical density (OD) reading in the BioPRYN test that was between 0% and 30% above the cutoff OD value were tested for serum P4 concentration. According to serum P4 concentration, cows were assigned to three categories: high (>4 ng/ml), medium (2–4 ng/ml) and low (<2 ng/ml) serum progesterone. The authors predicted a presumed (low) or possible (medium) late embryonic loss (LEL) or maintenance of the pregnancy (high). A total of 710 LELs were detected (17.4%) and 31.8% of them were predicted because of a low OD value at 30–36 days after insemination. Lower PSPB serum level significantly refers for LEL (p < 0.0001). The prediction rate for the true embryonic loss was 31.8% when OD cutoff from 0% to + 30% of cutoff was examined while it was 62.5% when the threshold was OD cutoff of 0% to 10% of cutoff. The authors conclude that BioPRYN was useful for prediction of a part of LEL in dairy cows and serum P4 concentration in these cows related to the rate of LEL.