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Procedure for Maximizing Oocyte Harvest for In Vitro Embryo Production in Small Ruminants
Author(s) -
Gibbons A,
Bonnet FP,
Cueto MI,
Catala M,
Salamone DF,
GonzalezBulnes A
Publication year - 2007
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2006.00802.x
Subject(s) - oocyte , andrology , follicular phase , biology , follicle , zoology , human fertilization , ovary , pregnancy rate , ovarian follicle , embryo , endocrinology , pregnancy , medicine , anatomy , genetics , microbiology and biotechnology
Contents Possible effects of repeated hormonal treatments and laparoscopic ovum pick‐up (LOPU) on the efficiency of oocyte recovery rate and quality were determined in sheep and goats. In six adult Merino sheep and five Criolla goats, ovarian status was synchronized by a prostaglandin F 2 α analogue and the insertion of an intravaginal sponge 48 h later. Follicle development was stimulated by a single dose of FSH (60 mg NIH‐FSH‐P1) plus a single dose of equine chorionic gonadotrophin (eCG; 300 UI). The first FSH/eCG doses were administered 48 h after the sponge insertion, being repeated every 4 days to complete a total of four treatments in sheep and three in goats. Follicles in both ovaries were categorized according to their diameter and follicular fluid was aspirated under laparoscopic observation without a vacuum pump. In sheep, during a 12‐day‐period, a total of 347 follicles were aspirated with a recovery rate of 46.9%. In goats, during an 8‐day‐period, 219 follicles were aspirated with a recovery rate of 45.6%. In both species, there were no significant differences in the number of aspirated follicles, oocyte recovery rate and good quality oocyte recovery rate. However, in sheep the oocyte recovery rate was higher for large follicles, whereas in goats no such effect was detected. In summary, current results indicate that retrieval of oocytes can be maximized, without affecting oocyte quality, by repeating ‘oneshot’ FSH/eCG regimes and LOPUs at intervals as short as 4 days.