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Effect of Progesterone and Oestradiol on Sperm–zona Binding and Acrosome Reaction in Bovine Spermatozoa after Thawing
Author(s) -
Lukoseviciute K,
Bizokas V,
Zilinskas H,
Januskauskas A
Publication year - 2007
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2006.00796.x
Subject(s) - acrosome reaction , andrology , sperm , acrosome , zona , chemistry , biology , medicine , immunology , virus , viral disease
Contents This study was conducted assess spermatozoa binding capacity to the oocyte in the presence of 0.1 or 0.5 μ g/ml progesterone (P4) or a combination of 0.5 μ g/ml P4 with 0.1 μ g/ml oestradiol (OE). The number of oocyte‐bound spermatozoa in the presence of progesterone was significantly higher (p < 0.05 to p < 0.001) when compared with the control samples, that were incubated in the absence of P4. Spermatozoa binding to the zona pellucida (ZP) of the oocyte were concentration‐dependent – significantly higher numbers of spermatozoa were bound in the presence of 0.5 μ g/ml P4, when compared with that of 0.1 μ g/ml P4. Oestradiol at 0.1 μ g/ml concentration used impaired the effect of progesterone‐mediated sperm–oocyte binding. The incidences of acrosome‐reacted (AR) spermatozoa bound to the ZP – following 0, 60, 120 and 180 min. incubation in the presence and absence of 1 μ g/ml progesterone was also assessed. Only at 180 min of incubation a higher (p < 0.001) incidence of the AR‐spermatozoa was found in sperm–ZP complexes incubated in the presence or absence of progesterone, being 56.5 ± 11.1 and 43.2 ± 8.8 % respectively. In conclusion, progesterone enhances the sperm–ZP binding capacity. Progesterone affects the incidences of AR on zona‐bound spermatozoa only after prolonged co‐culture.