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Capacitation of Frozen Thawed Buffalo Bull ( Bubalus bubalis ) Spermatozoa with Higher Heparin Concentrations
Author(s) -
Mehmood A,
Anwar M,
Saqlan Naqvi SM
Publication year - 2007
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2006.00794.x
Subject(s) - capacitation , acrosome reaction , heparin , andrology , ionophore , sperm , human fertilization , calcium , incubation , semen , acrosome , chemistry , bubalus , biology , biochemistry , medicine , anatomy , organic chemistry , ecology
Contents The present study was conducted to examine the effect of high heparin concentration on capacitation of buffalo spermatozoa with a short incubation time. Frozen thawed spermatozoa from three buffalo bulls were pooled and treated with either 50, 100 or 200 μ g/ml heparin for 30 min. Capacitation was evaluated by acrosome reaction of spermatozoa and in vitro fertilization rate (per cent cleavage rate, per cent cleavage index). Acrosome reaction was induced in heparin treated spermatozoa with calcium ionophore A23187 and staining was carried out with Coomassie G‐250 to evaluate the response as compared with control (0 heparin + calcium ionophore). Significantly higher percentage of acrosome reaction (AR) spermatozoa was noted after heparin treatment (36.8–48.2%) as compared with control (8.1% ; p < 0.05) but differences among the three heparin concentrations were non‐significant. However, a significantly higher in vitro fertilization rate was recorded in spermatozoa capacitated by 50 and 100 μ g/ml heparin (80.4 and 75.9% cleavage rate, respectively) as compared with 200 μ g/ml heparin (47.2% cleavage rate; p < 0.001). It is concluded that buffalo spermatozoa capacitated with 50–100 μ g/ml heparin had significantly higher ability to improve in vitro fertilization rate in buffalo.