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Morphometry Characterisation of Catalan Donkey Spermatozoa and Identification of Sperm Morphometric Subpopulations
Author(s) -
Miró J,
Flotats A,
Rivera MM,
Ocaña M,
Taberner E,
Peña A,
Rigau T
Publication year - 2006
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2006.00774_1_3.x
Subject(s) - donkey , semen , sperm , biology , andrology , anatomy , medicine , ecology
This study determined morphometric patterns of Catalan donkey spermatozoa. For this purpose, 32 ejaculates were collected from seven Catalan donkeys. Semen smears were stained with Harris’ Hematoxilin stain and analyzed with an ASMA system. VARCLUS and FASTCLUS procedures were applied to determine the most significant morphology parameters and the morphologic subpopulations respectively. Mean values of head morphometry were: length (LH) 5.45 ± 0.53 μm, width (WH) 2.68 ± 0.21 μm, area (AH) 11.56 ± 1.32 μm 2 , percentage of reacted acrosomes 45.2 ± 6.95%, FUN1 (LH/AH) 2.0 ± 0.21, FUN3 (4piS/PH 2 ) 0.3 ± 0.04. Mean values of midpiece were: width (WMP) 1.2 ± 1.08 μm, area (AMP) 3.2 ± 0.99 μm 2 , angle between head and midpiece (AHMP) 8.8 ± 17.46 degrees. Four different sperm morphometric subpopulations were identified. Subpopulation 1 (2.18% of spermatozoa) showed a WMP of 2.66 μm, an AMP of 39.96 μm 2 . Subpopulation 2 (72.78% of spermatozoa) showed a WMP of 1.00 μm, an AMP of 4.01 μm 2 . Subpopulation 3 (24.24% of spermatozoa) showed a WMP of 1.36 μm, an AMP of 14.11 μm 2 . Finally, subpopulation 4 (0.94% of spermatozoa) showed a WMP of 7.70 μm, an AMP of 171.53 μm 2 . It is noteworthy that four morphologic subpopulations were identified suggesting a possible relationship between these and the four motility subpopulations previously described (Miró et al. 2005, Theriogenology 63: 706–1716).