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Effects of Cryopreservation on the Expression of Glut‐3, Glut‐5 and As‐A Proteins in Iberian Boar Sperm Membranes
Author(s) -
Sancho S,
Casas I,
Pinart E,
Briz M,
GarciaGil N,
Bassols J,
Pruneda A,
Bussalleu E,
Yeste M,
Flores E,
RodríguezGil JE,
Ekwall H.,
RodríguezMartínez H.,
Bonet S
Publication year - 2006
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2006.00774_1_1.x
Subject(s) - acrosome , sperm , cryopreservation , andrology , boar , zona pellucida , semen , biology , chemistry , microbiology and biotechnology , embryo , oocyte , medicine
In order to determine the injure produced in boar spermatozoa through cryopreservation process, we analyzed the expression of the hexose transporters Glut‐3 and Glut‐5 and the zona pellucida binding protein As‐A (P68) in three different steps of the freezing‐thawed protocol: at 17°C (fresh BTS‐diluted semen, 1 : 2 v/v, step 1), at 5°C (after glycerol addition; step 2), and post‐thawing (step 3). All sperm analyses were carried out with immunogold techniques under electronic microscopy. For this study eight healthy post‐pubertal Iberian boars were submitted to a collection of twice per week through 3 months, evaluating two ejaculates from each boar. Glut‐3 maintains the expression in the acrosome region post‐thawing but not along the tail where is reduced. The expression of Glut‐5 and As‐A is majority located at the post‐acrosome region of the spermatozoa at step 1, but in step 2 and step 3 this expression is relocated to sperm tail area. In conclusion, while cryopreservation affects the localization and the expression of Glut‐3 and Glut‐5, its fertilizing capacity is not significantly reduced. The stabilization of boar semen at 5°C was found to be the most crucial step for sperm survival.