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Cryopreservation of Sheep Primordial Follicles
Author(s) -
Amorim CA,
Rondina D,
Lucci CM,
Giorgetti A,
De Figueiredo JR,
Gonçalves PBD
Publication year - 2007
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2006.00724.x
Subject(s) - cryoprotectant , cryopreservation , follicular phase , glycerol , andrology , ethylene glycol , biology , ovarian follicle , chemistry , endocrinology , embryo , biochemistry , medicine , microbiology and biotechnology , organic chemistry
Contents The aim of this study was to evaluate the efficiency of 1 M dimethylsulphoxide (DMSO), ethylene glycol (EG), propylene glycol (PROH) and glycerol (GLY) to cryopreserve primordial follicles. The first evaluation was performed soon after cryopreservation and the second evaluation after 4 days of in vitro culture, using the cryoprotectants that allowed the higher results (higher follicular survival rate) after cryopreservation. The results after follicular isolation (control) and cryopreservation using 1 M DMSO, EG, PROH and GLY showed that the mean number (± SEM) of live follicles per millilitre was 3204 (100%) ± 319.27, 2798 (87%) ± 239.14, 2492 (78%) ± 345.8, 448 (14%) ± 46.3 and 208 (7%) ± 75.26, respectively. Higher follicular survival was reported when DMSO and EG were used. Control follicles and follicles cryopreserved with these two cryoprotectants were cultured and the percentage of follicular survival was 55% (control), 42% (EG) and 34% (DMSO). Similar results were found between control and follicles cryopreserved with EG. In conclusion, 1 M EG is the most effective cryoprotectant to preserve primordial follicles isolated from ovaries of sheep.