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Role of Tumour Necrosis Factor α in Stimulation of Prostaglandins F 2 α and E 2 Release by Cultured Porcine Endometrial Cells
Author(s) -
Blitek A,
Ziecik AJ
Publication year - 2006
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2006.00716.x
Subject(s) - stromal cell , endocrinology , medicine , tumor necrosis factor alpha , stimulation , endometrium , secretion , biology , chemistry , andrology
Contents The present studies were undertaken to examine the effect of tumour necrosis factor (TNF) α on prostaglandins (PGs) F 2 α and E 2 release by cultured porcine endometrial cells harvested on days 13–16 after oestrus in comparison to stimulation with oxytocin (OT) and luteinizing hormone (LH). A time‐dependent effect of TNF α (10 ng/ml) on PGF 2 α release was observed in stromal and luminal epithelial cells. Moreover, TNF α increased PGF 2 α secretion from both endometrial cell types with effective concentrations of 1 (p < 0.05), 10 and 50 ng/ml (p < 0.01). The effect of TNF α (10 ng/ml) on endometrial PGF 2 α and PGE 2 release was compared with OT (100 nmol/l) and LH (100 ng/ml). All factors affected PGF 2 α secretion from stromal cells, however, the stimulation tended to be more potent after OT and LH (p < 0.01) than after TNF α (p < 0.05) treatment. In epithelial cells, only TNF α was able to stimulate PGF 2 α release (p < 0.001). PGE 2 secretion from stromal cells increased after incubation with TNF α and OT (p < 0.05). Only LH stimulated PGE 2 release from epithelium (p < 0.001), and its action was very effective when compared with TNF α or OT (p < 0.01). Summarizing, TNF α induces both PGs secretion from cultured porcine endometrium, but preferentially stimulates PGF 2 α release from luminal epithelial cells. Therefore, similarly to OT and LH, TNF α may be considered as a potential modulator of endometrial PGF 2 α production during luteolysis in the pig.