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Role of Two Glycosidases ( α ‐Mannosidase and β ‐ N ‐Acetylglucosaminidase) on In Vitro Bovine Embryonic Development *
Author(s) -
Tsiligianni TH,
Vandaele L,
Kruif A,
Soom A
Publication year - 2006
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2006.00657.x
Subject(s) - embryo , mannosidase , embryogenesis , in vitro , chemistry , glycoside hydrolase , andrology , enzyme , biology , microbiology and biotechnology , biochemistry , medicine
Contents Glycosidases are enzymes that might play a role in embryonic development. The aims of the present project were to evaluate if bovine in vitro produced embryos: (1) release β ‐ N ‐acetylglucosaminidase ( β ‐NAGASE) and α ‐mannosidase in culture medium and (2) to investigate if these glycosidases may be used as markers of embryo quality. Bovine embryos were obtained using routine methods for IVM, IVF and IVC. Two experiments were done [(experiment 1: culture of embryos in the same droplet until day 7 and experiment 2: separation and transfer of embryos to new droplets at the morula stage (day 6)]. Samples were collected on day 7 (experiment 1) and on days 6 and 7 (experiment 2). The results of the present study are summarized as follow: (i) Embryos release both glycosidases. (ii) The activity of both glycosidases was significantly lower (p < 0.05) in droplets with degenerate embryos compared to droplets without degenerate embryos. (iii) The activity of β ‐NAGASE was higher in droplets which contained morulae compared to droplets without morulae. In conclusion, embryos release both glucosidases during their development, while degenerate embryos release less β ‐NAGASE and α ‐mannosidase compared to good embryos. Furthermore, β ‐NAGASE secretion seems to be related to retarded morulae.