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Effects of β ‐OH Butyrate on Bovine Granulosa and Theca Cell Function in Vitro
Author(s) -
Vanholder T,
Leroy JLMR,
Soom A,
Coryn M,
Kruif A,
Opsomer G
Publication year - 2006
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2006.00634.x
Subject(s) - theca , in vitro , chemistry , butyrate , andrology , function (biology) , medicine , endocrinology , biology , ovary , microbiology and biotechnology , biochemistry , fermentation
Contents In this study, the effects of β ‐OH butyrate (BHB) levels, associated with a negative energy balance, on bovine granulosa and theca cell function were investigated in vitro . Granulosa and theca cells of healthy large follicles (>8 mm), obtained from slaughterhouse ovaries, were cultured in serum free medium containing 0, 0.5, 1 or 1.5 m m BHB and 3 m m glucose, to mimic the situation in the early postpartum dairy cow. Hormone concentrations (progesterone, oestradiol‐17 β and/or androstenedione) in spent medium and cell numbers were measured after 48 h of culture. No effects of BHB on theca cell numbers or on steroid production were observed. In granulosa cells, all BHB treatments evenly increased cell numbers (p < 0.05), while they reduced progesterone and oestradiol‐17 β production per cell (p < 0.05). These effects may be attributed to the use of BHB as energy source which is however differently metabolized than glucose. Conclusively, in the presence of physiological glucose concentrations BHB can modulate granulosa but not theca cell function in vitro .