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The Activity of Matrix Metalloproteinase‐2 and ‐9 in Serum of Pregnant and Non‐Pregnant Bitches
Author(s) -
SchäferSomi S,
Ali Aksoy O,
Patzl M,
Findik M,
ErünalMaral N,
Beceriklisoy HB,
Polat B,
Aslan S
Publication year - 2005
Publication title -
reproduction in domestic animals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.546
H-Index - 66
eISSN - 1439-0531
pISSN - 0936-6768
DOI - 10.1111/j.1439-0531.2004.00552.x
Subject(s) - matrix metalloproteinase , endocrinology , enzyme , enzyme assay , medicine , andrology , pregnancy , chemistry , biology , biochemistry , genetics
Contents The aim of this study was to investigate, whether the activity of matrix metalloproteinase (MMP)‐2 and ‐9 in the serum of pregnant and non‐pregnant bitches differs significantly. For this purpose, 81 blood samples were taken from pregnant bitches at days 5–13, 15–21, 24–31, 34–40 and 41–50 after mating, and 51 samples from non‐pregnant animals at corresponding times. Relative enzyme activity, calculated as the percentage of serum enzyme activity on enzyme activity in a control sample, was determined with a commercially available assay after activation of serum MMPs with p‐aminophenylmercuric acetate (APMA). In addition, serum oestradiol (E 2 ) and progesterone (P 4 ) concentrations were measured with an enzymeimmunoassay (EIA). In the pregnant bitches, at days 5–13 and 15–21 after mating, the mean activity of both MMPs was significantly higher than in non‐pregnant animals (28.5% vs 24.5% and 27.7% vs 22.6%; p < 0.01). Moreover, in the pregnant bitches, significant correlations were detected between the serum enzyme activity and the serum concentrations of E 2 (−0.900; p < 0.05) and P 4 (+0.667; p < 0.05).